4LZD
Human DNA polymerase mu- Apoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2012-09-03 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 124.192, 124.192, 50.407 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.856 - 1.849 |
Rwork | 0.181 |
R-free | 0.22830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2IHM (molecule B) was used as the starting molecule for molecular replacement for a lower resolution structure which was then used as the starting model for the current higher resolution structure. |
RMSD bond length | 0.011 |
RMSD bond angle | 1.280 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
High resolution limit [Å] | 1.849 | 3.990 | 1.850 |
Rmerge | 0.037 | 0.414 | |
Number of reflections | 34121 | ||
<I/σ(I)> | 16.4 | ||
Completeness [%] | 99.3 | 99.5 | 96.4 |
Redundancy | 9 | 13.5 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Crystals were grown by mixing 1 uL of concentrated protein (10.7mg/mL) with 1 uL of mother liquor (50 mM imidazole pH 8, 0.8M sodium citrate) at room temperature, using the hanging drop vapor diffusion technique. The crystals reached usable size within 24 hours, and were then directly transferred to a cryoprotectant solution containing 50mM imidazole pH 8, 75mM NaCl, 1M sodium citrate, 10% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 295K |