4LZ9

Structure of MATE multidrug transporter DinF-BH in complex with R6G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	90.181, 93.887, 102.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.700
R-factor	0.3
Rwork	0.290
R-free	0.30000
Structure solution method	AB INITIO PHASING
RMSD bond length	0.011
RMSD bond angle	2.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000
High resolution limit [Å]	3.700	3.700
Number of reflections	9220
Completeness [%]	95.0	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	PEG, NaCl, pH 8.5, VAPOR DIFFUSION, temperature 293K