4LYX
five minutes iron loaded frog M ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.2398 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.180, 184.180, 184.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.220 - 1.230 |
| R-factor | 0.16135 |
| Rwork | 0.161 |
| R-free | 0.17521 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ka3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.289 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 22.330 | 22.330 | 1.300 |
| High resolution limit [Å] | 1.230 | 3.890 | 1.230 |
| Rmerge | 0.050 | 0.029 | 0.424 |
| Number of reflections | 77177 | ||
| <I/σ(I)> | 15.2 | 43 | 2.3 |
| Completeness [%] | 99.7 | 99.4 | 98.4 |
| Redundancy | 6.4 | 7.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 1.6-2M magnesium chloride, 0.1M bicine , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
