4LYQ
Crystal Structure of Glycoside Hydrolase Family 5 Mannosidase from Rhizomucor miehei, E202A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-19 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.173, 76.549, 54.836 |
Unit cell angles | 90.00, 113.36, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.12685 |
Rwork | 0.125 |
R-free | 0.16326 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uuq |
RMSD bond length | 0.026 |
RMSD bond angle | 2.177 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.600 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.044 | |
Number of reflections | 31831 | |
Completeness [%] | 94.5 | 63.1 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 16% PEG 4000, 0.1M HEPES buffer pH 7.5, 5% mannotriose, VAPOR DIFFUSION, SITTING DROP, temperature 293K |