4LYP
Crystal Structure of Glycoside Hydrolase Family 5 Mannosidase from Rhizomucor miehei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-21 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.646, 171.991, 55.137 |
| Unit cell angles | 90.00, 104.60, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.280 |
| R-factor | 0.12102 |
| Rwork | 0.119 |
| R-free | 0.15344 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.056 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 86.000 | 20.000 | 1.300 |
| High resolution limit [Å] | 1.280 | 3.470 | 1.280 |
| Rmerge | 0.052 | ||
| Number of reflections | 205153 | ||
| Completeness [%] | 87.6 | 27.1 | 91.4 |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 16% PEG 4000, 0.2 M guanidine hydrochloride, 0.1M HEPES buffer pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
