4LYO
CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN NEAT ACETONITRILE, THEN BACK-SOAKED IN WATER
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 285 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.430, 79.430, 37.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.050 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.19300 |
| Structure solution method | DIRECT REFINEMENT |
| Starting model (for MR) | 6lyt |
| RMSD bond length | 0.013 |
| RMSD bond angle | 23.900 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 80.000 |
| High resolution limit [Å] | 1.930 |
| Rmerge | 0.070 |
| Total number of observations | 47160 * |
| Number of reflections | 7540 |
| <I/σ(I)> | 13.3 |
| Completeness [%] | 93.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.1 * | 24-25 * | PROTEIN WAS CRYSTALLIZED FROM SODIUM ACETATE BUFFER (PH 4.3) CONTAINING 6.0% (W/V) SODIUM CHLORIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | drop | HAc-NaAc | 10 (mM) | |
| 3 | 1 | reservoir | HAc-NaAc | 0.2 (M) | |
| 4 | 1 | reservoir | 6.0 (%(w/v)) |
