4LY7
Ancestral RNase H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1159 |
Spacegroup name | P 61 |
Unit cell lengths | 82.379, 82.379, 44.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.189 - 1.360 |
R-factor | 0.1279 |
Rwork | 0.126 |
R-free | 0.15590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rn2 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.329 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.189 | 1.410 |
High resolution limit [Å] | 1.360 | 1.360 |
Number of reflections | 37194 | |
<I/σ(I)> | 26.1 | 2.7 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 7.6 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 20% PEG 3350, 30mM lithium sulfate, 1mM TCEP, 100mM Bis-Tris pH 6.5, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |