4LXY

Crystal structure WlaRD, a sugar 3N-formyl transferase in the presence of dTDP and 10-N-Formyl-THF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-10
Detector	Bruker Platinum 135
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	96.337, 65.858, 134.600
Unit cell angles	90.00, 109.88, 90.00

Refinement procedure

Resolution	48.210 - 1.640
R-factor	0.19095
Rwork	0.189
R-free	0.22564
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lxq
RMSD bond length	0.011
RMSD bond angle	2.078
Data reduction software	APEX
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.740
High resolution limit [Å]	1.640	1.640
Rmerge	0.058	0.364
Number of reflections	93525
<I/σ(I)>	14.6	2.2
Completeness [%]	96.2	86.6
Redundancy	4	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	11-15% PEG 8000, 200 mM NaCl, 5 mM TDP, 5 mM N-5-Formyl-THF, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K