4LXU
dTdp-Fuc3N and 5-N-Formyl-THF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-12 |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.534, 64.884, 134.458 |
| Unit cell angles | 90.00, 110.67, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.400 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lxq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.138 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.055 | 0.129 |
| Number of reflections | 145264 | |
| <I/σ(I)> | 48 | 9.5 |
| Completeness [%] | 95.5 | 84.8 |
| Redundancy | 4.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 295 | 11-15% PEG 8000, 200 mM NaCl, 5 mM N-5-formyl-THF, 5 mM TDP-Fuc3N, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
