4LXO
Crystal structure of 9,10Fn3-elegantin chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1,0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.115, 75.115, 175.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.400 - 1.420 |
| R-factor | 0.16402 |
| Rwork | 0.163 |
| R-free | 0.19305 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fnf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.653 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.470 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.036 | 0.436 |
| Number of reflections | 95355 | |
| <I/σ(I)> | 49.4 | 3.1 |
| Completeness [%] | 97.4 | 91.74 |
| Redundancy | 7.8 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.2M calcium acetate, 7%(w/v) PEG10000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
