4LX3
Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2012-05-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.029, 61.199, 103.997 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.800 - 1.500 |
| R-factor | 0.1673 |
| Rwork | 0.167 |
| R-free | 0.19430 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.352 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.800 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 22916 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 20mM Tris-HCl, 100mM NaCl, 2mM Dithiothreitol, pH 8.0, VAPOR DIFFUSION, SITTING DROP |
