4LX1

Crystal structure of Myo5a globular tail domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-06
Detector	PSI PILATUS 6M
Wavelength(s)	0.980110
Spacegroup name	P 21 21 21
Unit cell lengths	78.080, 94.580, 135.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 1.870
R-factor	0.166
Rwork	0.164
R-free	0.19940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lx0
RMSD bond length	0.011
RMSD bond angle	1.245
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	1.980
High resolution limit [Å]	1.870	1.870
Rmerge		0.360
Number of reflections	83459
<I/σ(I)>		4.89
Completeness [%]	99.9	99.3
Redundancy		7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	MES pH6 12% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K