4LW9
Crystal structure of Vibrio cholera major pseudopilin EpsG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0712 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.610, 70.015, 131.544 |
| Unit cell angles | 90.00, 103.29, 90.00 |
Refinement procedure
| Resolution | 29.540 - 1.900 |
| R-factor | 0.20468 |
| Rwork | 0.202 |
| R-free | 0.24527 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.323 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.540 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 117230 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 6 mM zinc sulfate, 60 mM MES pH 6.5, 15% PEG MME 550, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
