4LW6

Crystal structure of catalytic domain of Drosophila beta1,4galactosyltransferase 7 complex with xylobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-07-17
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	81.943, 81.943, 133.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.004 - 2.400
R-factor	0.1789
Rwork	0.177
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lw6
RMSD bond length	0.014
RMSD bond angle	1.557
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	17496
<I/σ(I)>	21.2	2
Completeness [%]	94.8	77
Redundancy	7.6	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	280	100 mM HEPES, 5% PEG 6000, 10% MPD, 100 mM Serinol, 10 mM xylobiose, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 280K