4LVM

MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (23nt). Mn-bound crystal structure at pH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97243
Spacegroup name	P 21 21 2
Unit cell lengths	67.340, 130.120, 87.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.400 - 3.100
R-factor	0.25696
Rwork	0.255
R-free	0.29359
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.409
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.400
High resolution limit [Å]	3.100
Rmerge	0.092
Number of reflections	13500
<I/σ(I)>	9.4
Completeness [%]	98.7
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	20% PEG 8000, 02.M MgAc(4H20), 0.1M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K