4LVJ

MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt). Mn-bound crystal structure at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 61 2 2
Unit cell lengths	112.020, 112.020, 90.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.220 - 2.170
R-factor	0.20322
Rwork	0.201
R-free	0.24116
RMSD bond length	0.012
RMSD bond angle	1.784
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000	2.290
High resolution limit [Å]	2.170	2.170
Rmerge	0.150
Number of reflections	16928
<I/σ(I)>	7.3
Completeness [%]	98.2	100
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	21% PEG 6000, 0.1M Sodium Acetate pH4.6. Cryo-protecting/soaking at pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K