4LVI

MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt). Mn-bound crystal structure at pH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97950
Spacegroup name	P 61 2 2
Unit cell lengths	111.060, 111.060, 90.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.100 - 1.900
R-factor	0.20768
Rwork	0.206
R-free	0.24774
RMSD bond length	0.014
RMSD bond angle	1.740
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.100	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.109
Number of reflections	25154
<I/σ(I)>	8.2
Completeness [%]	99.8	99.6
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	20-22% PEG 6000, 0.1M Sodium Acetate, pH4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K