4LVI
MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt). Mn-bound crystal structure at pH 4.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97950 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 111.060, 111.060, 90.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.100 - 1.900 |
R-factor | 0.20768 |
Rwork | 0.206 |
R-free | 0.24774 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.740 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.100 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.109 | |
Number of reflections | 25154 | |
<I/σ(I)> | 8.2 | |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 20-22% PEG 6000, 0.1M Sodium Acetate, pH4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |