4LVH

Insight into highly conserved H1 subtype-specific epitopes in influenza virus hemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	72.751, 237.814, 94.230
Unit cell angles	90.00, 110.31, 90.00

Refinement procedure

Resolution	43.490 - 2.800
R-factor	0.2338
Rwork	0.232
R-free	0.27410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f15
RMSD bond length	0.018
RMSD bond angle	2.242
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge		0.790
Number of reflections	63466
<I/σ(I)>		1
Completeness [%]	86.8	81.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	20% PEG 3350, 200mM potassium iodide, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K