4LUM
The crystal structure of the P132V mutant of Pyrococcus furiosus phosphoglucose isomerase in complex with manganese and fructose-6- phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96860 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.940, 45.010, 48.690 |
| Unit cell angles | 87.81, 89.86, 75.47 |
Refinement procedure
| Resolution | 18.490 - 1.790 |
| R-factor | 0.17577 |
| Rwork | 0.173 |
| R-free | 0.22008 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x7n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.265 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.490 | 1.840 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.086 | 0.349 |
| Number of reflections | 33074 | |
| <I/σ(I)> | 4.3 | 2.2 |
| Completeness [%] | 95.7 | 94 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 0.2 M sodium nitrate, 0.1 M Bis Tris Propane PH6.5, 20% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
