4LUK
The crystal structure of the P132A, Y133G mutant of Pyrococcus furiosus phosphoglucose isomerase in complex with manganese and 5-phospho-D-arabinonate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-05-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97630 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.850, 43.280, 58.450 |
Unit cell angles | 90.00, 122.90, 90.00 |
Refinement procedure
Resolution | 28.920 - 1.410 |
R-factor | 0.15336 |
Rwork | 0.150 |
R-free | 0.21388 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x7n |
RMSD bond length | 0.006 |
RMSD bond angle | 1.064 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.920 | 1.440 |
High resolution limit [Å] | 1.410 | 1.410 |
Rmerge | 0.036 | 0.349 |
Number of reflections | 32797 | |
<I/σ(I)> | 13 | 2.1 |
Completeness [%] | 91.2 | 55.4 |
Redundancy | 3.1 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 290 | 0.35 M MgCl2, 0.1 M sodium acetate PH 5.5 and 19% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 290K |