4LUH
Complex of ovine serum albumin with 3,5-diiodosalicylic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 118.530, 118.530, 120.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.220 - 2.200 |
| R-factor | 0.17212 |
| Rwork | 0.171 |
| R-free | 0.20887 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4luf |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.170 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.099 | 0.084 |
| Number of reflections | 50067 | |
| <I/σ(I)> | 2.96 | 2.96 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.44 | 7.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 70% Tacsimate, 4% PPG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
