4LUF

Crystal Structure of Ovine Serum Albumin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-14
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 32 2 1
Unit cell lengths	118.390, 118.390, 120.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.190 - 2.300
R-factor	0.177
Rwork	0.176
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f5s
RMSD bond length	0.018
RMSD bond angle	2.138
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.096	0.902
Number of reflections	43652
<I/σ(I)>	12.11	2
Completeness [%]	99.4	99.8
Redundancy	3.69	3.73

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	293	Tacsimate 70%, 4% PPG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K