4LUA
Crystal structure of N-acetyltransferase from Staphylococcus aureus Mu50
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.939, 84.939, 49.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.860 - 1.600 |
| R-factor | 0.1798 |
| Rwork | 0.178 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g8w |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.754 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.310 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.069 | |
| Number of reflections | 24239 | |
| <I/σ(I)> | 13.8 | 5.2 |
| Completeness [%] | 99.8 | 96.5 |
| Redundancy | 13.8 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 296 | 2.25M sodium formate,100mM sodium acetate trihydrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
