4LU4
Crystal Structure of the N-terminal Fic Domain of a Putative Cell Filamentation protein (VirB-translocated Bep effector protein) from Bartonella quintana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.560, 43.630, 88.760 |
| Unit cell angles | 90.00, 91.53, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.000 |
| R-factor | 0.18326 |
| Rwork | 0.180 |
| R-free | 0.23512 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jk8 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.749 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 | |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.114 | 0.071 | 0.386 |
| Number of reflections | 30109 | ||
| <I/σ(I)> | 8.18 | 13.42 | 3.13 |
| Completeness [%] | 99.7 | 96.3 | 99.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | MORPHEUS_b11: 30mM each NaF, NaBr, NaI, 100mM Tris base/Bicine, 15% Glycerol, 15% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
