4LU4
Crystal Structure of the N-terminal Fic Domain of a Putative Cell Filamentation protein (VirB-translocated Bep effector protein) from Bartonella quintana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.560, 43.630, 88.760 |
Unit cell angles | 90.00, 91.53, 90.00 |
Refinement procedure
Resolution | 19.920 - 2.000 |
R-factor | 0.18326 |
Rwork | 0.180 |
R-free | 0.23512 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jk8 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.749 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 | |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.114 | 0.071 | 0.386 |
Number of reflections | 30109 | ||
<I/σ(I)> | 8.18 | 13.42 | 3.13 |
Completeness [%] | 99.7 | 96.3 | 99.9 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | MORPHEUS_b11: 30mM each NaF, NaBr, NaI, 100mM Tris base/Bicine, 15% Glycerol, 15% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |