4LU1
Crystal structure of the uncharacterized Maf protein YceF from E. coli, mutant D69A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-18 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.651, 52.834, 129.275 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.420 - 1.920 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jhc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.272 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.950 |
| High resolution limit [Å] | 1.920 | 5.210 | 1.920 |
| Rmerge | 0.066 | 0.034 | 0.644 |
| Number of reflections | 27172 | ||
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 99.3 | 98.4 | 95.1 |
| Redundancy | 7.1 | 6.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1M MES pH 6.0, 20% PEG10K, 1/10 papain in protein, temperature 291K, VAPOR DIFFUSION, HANGING DROP |
