4LU0
Crystal structure of 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-06-25 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.270, 95.100, 145.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.690 - 2.800 |
| R-factor | 0.20866 |
| Rwork | 0.206 |
| R-free | 0.26717 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g7v |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.470 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.800 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.243 |
| Number of reflections | 27850 |
| <I/σ(I)> | 6.7 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M calcium acetate, and 0.1 M sodium cacodylate pH 6.5, 18% (w/v) polyethylene glycol 8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
