4LTR
Bacterial sodium channel, His245Gly mutant, I222 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.70192 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 157.205, 161.384, 165.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 5.800 |
| R-factor | 0.26519 |
| Rwork | 0.262 |
| R-free | 0.31762 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lto |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.966 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 6.110 |
| High resolution limit [Å] | 5.800 | 5.800 |
| Rmerge | 0.130 | 4.181 |
| Number of reflections | 5978 | |
| <I/σ(I)> | 0.7 | 0.7 |
| Completeness [%] | 98.7 | 98.7 |
| Redundancy | 13.1 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 28% PEG400, 100 mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
