4LTM

Crystal structures of NADH:FMN oxidoreductase (EMOB) - FMN complex

Replaces:  2VZH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-12-05
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 64 2 2
Unit cell lengths	101.270, 101.270, 130.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.193 - 2.497
Rwork	0.198
R-free	0.20390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ltd
RMSD bond length	0.007
RMSD bond angle	1.149
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.200
High resolution limit [Å]	2.497	2.497
Number of reflections	14221

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	10 mg/mL EmoB in 20 mM sodium acetate, pH 5, 1 mM EDTA, 1 mM DTT mixed with an equal volume of reservoir solution (0.1 M MES, pH 6.5, 1.6 M ammonium sulfate, 10% dioxane), VAPOR DIFFUSION, HANGING DROP, temperature 277K