4LTH
Dehydration/Rehydration of a Nucleic Acid system containing a Polypyridyl Ruthenium Complex at 97% relative humidity (3/7)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2012-12-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.8266 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 42.980, 42.980, 39.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.390 - 1.600 |
R-factor | 0.14646 |
Rwork | 0.145 |
R-free | 0.17432 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qrn |
RMSD bond length | 0.017 |
RMSD bond angle | 2.995 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.390 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 4564 | |
Completeness [%] | 88.6 | 91.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | 1ul 1mM d(TCGGCGCCGA)2, 1ul 4mM lambda-[Ru(TAP)2(dppz)]2+, 6ul 12mM spermine, 10% MPD, 40mM sodium cacodylate, 80mM KCl, 20mM BaCl2. Equilibriated against 1ml 35% MPD. Crystal was rehydrated to 97% relative humidity on the beamline using the HC1b., pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |