Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0332, 0.97925, 0.97942, 0.91162 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 100.925, 100.925, 129.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.241 - 2.186 |
R-factor | 0.1708 |
Rwork | 0.169 |
R-free | 0.19180 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.167 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | X-PLOR |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.241 | |
High resolution limit [Å] | 2.186 | 2.186 |
Number of reflections | 17012 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10 mg/mL EmoB in 20 mM sodium acetate, pH 5, 1 mM EDTA, 1 mM DTT mixed with an equal volume of reservoir solution (0.1 M MES, pH 6.5, 1.6 M ammonium sulfate, 10% dioxane), VAPOR DIFFUSION, HANGING DROP, temperature 277K |