4LT9
Crystal Structure of Engineered Protein, Northeast Structural Genomics Consortium Target OR404
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.287, 73.333, 80.738 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.667 - 2.150 |
R-factor | 0.219 |
Rwork | 0.218 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 4A2R |
RMSD bond length | 0.008 |
RMSD bond angle | 1.530 |
Data reduction software | XDS |
Data scaling software | RAPD |
Phasing software | BALBES |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.118 | 0.012 |
Number of reflections | 24498 | |
<I/σ(I)> | 16.8 | 2.4 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 10.5 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch crystallization under oil | 8 | 277 | 24% PEG 20000, 0.1M potassium chloride, 0.1M TrisHCl, pH 8.0, Microbatch crystallization under oil, temperature 277K |