4LSX
Plant steroid receptor ectodomain bound to brassinolide and SERK1 co-receptor ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.999870 |
| Spacegroup name | P 65 |
| Unit cell lengths | 69.896, 69.896, 873.547 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.530 - 3.302 |
| R-factor | 0.2484 |
| Rwork | 0.246 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4LSA AND 4LSC |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.883 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1334)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.530 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.121 | 0.750 |
| Number of reflections | 35235 | |
| <I/σ(I)> | 12.16 | 2.34 |
| Completeness [%] | 98.1 | 96.4 |
| Redundancy | 6.67 | 6.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 298 | 22% PEG3350, 0.2 M sodium chloride, 0.1 M Bis-Tris, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
