4LSX

Plant steroid receptor ectodomain bound to brassinolide and SERK1 co-receptor ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999870
Spacegroup name	P 65
Unit cell lengths	69.896, 69.896, 873.547
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.530 - 3.302
R-factor	0.2484
Rwork	0.246
R-free	0.28480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4LSA AND 4LSC
RMSD bond length	0.003
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1334))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.530	3.500
High resolution limit [Å]	3.300	3.300
Rmerge	0.121	0.750
Number of reflections	35235
<I/σ(I)>	12.16	2.34
Completeness [%]	98.1	96.4
Redundancy	6.67	6.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	298	22% PEG3350, 0.2 M sodium chloride, 0.1 M Bis-Tris, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K