4LSV
Crystal structure of broadly and potently neutralizing antibody 3BNC117 in complex with HIV-1 clade C C1086 gp120
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-14 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 191.615, 191.615, 103.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.320 - 3.000 |
R-factor | 0.262 |
Rwork | 0.260 |
R-free | 0.29900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.589 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.020 |
High resolution limit [Å] | 2.840 | 2.840 |
Rmerge | 0.091 | 0.524 |
Number of reflections | 22670 | |
<I/σ(I)> | 17.46 | 3.32 |
Completeness [%] | 98.5 | 95.5 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 293 | 0.1M imidazole, 11% PEG 3350, 0.5M NaCl, 0.2M MgCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |