4LSI
Ion selectivity of OmpF porin soaked in 0.3M KBr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91956 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.094, 116.559, 53.592 |
| Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
| Resolution | 32.740 - 2.089 |
| R-factor | 0.19252 |
| Rwork | 0.190 |
| R-free | 0.24534 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2omf |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.993 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.089 | 2.089 |
| Rmerge | 0.117 | 0.654 |
| Number of reflections | 71693 | |
| <I/σ(I)> | 13.6 | 1.78 |
| Completeness [%] | 98.7 | 99.6 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50% PEG200, 0.1 M sodium cacodylate, 0.2 M magnesium chloride, pH 6.5, crystals soaked in 0.3 M potassium bromide, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
