4LSC

Isolated SERK1 co-receptor ectodomain at high resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97794
Spacegroup name	P 61
Unit cell lengths	72.630, 72.630, 80.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.673 - 1.529
R-factor	0.1679
Rwork	0.167
R-free	0.17720
Structure solution method	MR-SAD
Starting model (for MR)	1ogq
RMSD bond length	0.009
RMSD bond angle	1.421
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1334))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.700	1.570
High resolution limit [Å]	1.529	1.529
Rmerge	0.075	1.010
Number of reflections	36620
<I/σ(I)>	16.12	2.15
Completeness [%]	100.0	99.9
Redundancy	10.2	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	1.7 M sodium malonate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	5	298	1.7 M sodium malonate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K