4LS4

Crystal structure of L66S mutant toxin from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-12
Detector	RIGAKU SATURN A200
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	38.267, 47.792, 97.246
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.550 - 1.660
R-factor	0.17499
Rwork	0.172
R-free	0.22424
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	2.122
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.660
Number of reflections	21724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K