4LRW
Crystal Structure of K-Ras G12C (cysteine-light), GDP-bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9999 |
Spacegroup name | P 3 |
Unit cell lengths | 82.862, 82.862, 38.397 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.920 - 2.151 |
R-factor | 0.1828 |
Rwork | 0.180 |
R-free | 0.20430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.558 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.2.4) |
Refinement software | PHENIX (dev_1402) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.128 | 0.354 |
Number of reflections | 16023 | |
<I/σ(I)> | 12.884 | 5.48 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 30% PEG4000, 0.2M MgSO4, 0.1M TRIS, pH 8.0, vapor diffusion, hanging drop, temperature 293K |