4LRV
Crystal structure of DndE from Escherichia coli B7A involved in DNA phosphorothioation modification
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.845, 97.306, 218.532 |
| Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.20306 |
| Rwork | 0.201 |
| R-free | 0.24166 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.105 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 62884 |
| Completeness [%] | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.6 | 277 | 1.5M sodium citrate (pH 6.6), 1M cesium chloride, VAPOR DIFFUSION, temperature 277K |
