4LR4

Crystal structure of a putative secreted protein (EUBREC_3654) from Eubacterium rectale at 2.43 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91837, 0.97864, 0.97805
Spacegroup name	P 1 21 1
Unit cell lengths	98.016, 106.733, 115.078
Unit cell angles	90.00, 92.45, 90.00

Refinement procedure

Resolution	48.963 - 2.430
R-factor	0.1876
Rwork	0.186
R-free	0.21640
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE (July 4, 2012)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.963		2.520
High resolution limit [Å]	2.430	5.210	2.430
Rmerge	0.102	0.045	0.777
Number of reflections	87733	9030	9088
<I/σ(I)>	9.58	21.6	1.9
Completeness [%]	98.1	97.9	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.83	277	1.5M ammonium dihydrogen phosphate, 20.0% Glycerol, 0.01M magnesium chloride, 0.1M TRIS pH 7.83, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K