4LQZ
Crystal structure of a DUF4909 family protein (SAV1798) from Staphylococcus aureus subsp. aureus Mu50 at 1.92 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97987,0.91837,0.9793 |
Spacegroup name | P 4 |
Unit cell lengths | 60.352, 60.352, 37.388 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.675 - 1.920 |
R-factor | 0.1967 |
Rwork | 0.195 |
R-free | 0.23600 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | XSCALE (July 4, 2012) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.675 | 42.675 | 1.990 |
High resolution limit [Å] | 1.920 | 4.130 | 1.920 |
Rmerge | 0.067 | 0.029 | 0.651 |
Number of reflections | 10252 | 1079 | 918 |
<I/σ(I)> | 12.3 | 37.3 | 1.72 |
Completeness [%] | 97.8 | 97.6 | 86.4 |
Redundancy | 3.68 | 3.23 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 50.00% polyethylene glycol 200, 0.200M sodium chloride, 0.1M phosphate-citrate pH 4.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |