4LQV

Thrirty minutes iron loaded frog M ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-03
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.954
Spacegroup name	F 4 3 2
Unit cell lengths	184.084, 184.084, 184.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.230 - 1.540
R-factor	0.16886
Rwork	0.168
R-free	0.19270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ka3
RMSD bond length	0.005
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.230	42.230	1.620
High resolution limit [Å]	1.540	4.870	1.540
Rmerge	0.148	0.148	0.278
Number of reflections	39348
<I/σ(I)>	9.5	13.3	6.1
Completeness [%]	98.8	92.7	100
Redundancy	8.5	8.5	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	1.6-2M magnesium chloride, 0.1M bicine , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K