4LQU

1.60A resolution crystal structure of a superfolder green fluorescent protein (W57G) mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	96.065, 97.252, 98.163
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.342 - 1.600
R-factor	0.1562
Rwork	0.155
R-free	0.18240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.009
RMSD bond angle	1.075
Data reduction software	XDS
Data scaling software	SCALA (0.1.29)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (dev_1405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.087	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.075	0.955
Total number of observations		39027
Number of reflections	121582
<I/σ(I)>	15.3	2
Completeness [%]	100.0	100
Redundancy	6.6	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	25% (v/v) PEG 3350, 0.1 M bis-tris, pH 5.5, vapor diffusion, temperature 293K