4LQR
Structure of CBM32-3 from a family 31 glycoside hydrolase from Clostridium perfringens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 93.15 |
Detector technology | CCD |
Collection date | 2009-10-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.766, 52.562, 70.169 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.960 - 1.580 |
R-factor | 0.1626 |
Rwork | 0.161 |
R-free | 0.19400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j1a |
RMSD bond length | 0.024 |
RMSD bond angle | 2.248 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.640 |
High resolution limit [Å] | 1.580 | 3.400 | 1.580 |
Rmerge | 0.042 | 0.035 | 0.084 |
Number of reflections | 20155 | ||
Completeness [%] | 98.9 | 98.4 | 91 |
Redundancy | 5.4 | 7.1 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 10000, 100mM HEPES 50mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |