4LQQ
Crystal structure of the Cbk1(T743E)-Mob2 kinase-coactivator complex in crystal form B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-05-28 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.740, 139.060, 187.580 |
Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
Resolution | 46.490 - 3.600 |
R-factor | 0.2621 |
Rwork | 0.258 |
R-free | 0.31940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.826 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.490 | 3.690 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 23529 | |
Completeness [%] | 98.8 | 91.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under oil | 5.5 | 296 | four fold excess pepSSD1, 25% PEG 20,000 buffered with 0.1M Na-citrate, pH 5.5, Microbatch under oil, temperature 296K |