4LQQ
Crystal structure of the Cbk1(T743E)-Mob2 kinase-coactivator complex in crystal form B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.740, 139.060, 187.580 |
| Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
| Resolution | 46.490 - 3.600 |
| R-factor | 0.2621 |
| Rwork | 0.258 |
| R-free | 0.31940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.826 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.490 | 3.690 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 23529 | |
| Completeness [%] | 98.8 | 91.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil | 5.5 | 296 | four fold excess pepSSD1, 25% PEG 20,000 buffered with 0.1M Na-citrate, pH 5.5, Microbatch under oil, temperature 296K |
