4LQP
Crystal structure of the Cbk1(T743E)-Mob2 kinase-coactivator complex, in crystal form A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-05-28 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 129.380, 129.380, 231.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.590 - 4.500 |
R-factor | 0.2887 |
Rwork | 0.288 |
R-free | 0.31040 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.665 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.591 | 4.660 |
High resolution limit [Å] | 4.500 | 4.500 |
Number of reflections | 12238 | |
Completeness [%] | 98.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under oil | 5.5 | 296 | 25% PEG 20,000 buffered with 0.1M Na-citrate, pH 5.5, Microbatch under oil, temperature 296K |