4LQL

Crystal structure of L-arabinose isomerase from Lactobacillus fermentum CGMCC2921

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	173
Detector technology	CCD
Collection date	2012-05-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.054
Spacegroup name	P 21 21 21
Unit cell lengths	85.201, 184.831, 186.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.849 - 3.232
R-factor	0.3203
Rwork	0.314
R-free	0.43160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ajt
RMSD bond length	0.011
RMSD bond angle	1.622
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.590
High resolution limit [Å]	3.230	3.230
Rmerge	0.102
Number of reflections	47550
Completeness [%]	99.0	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1M Bis-Tris, 25% PEGMME5000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K