4LQH
High resolution structure of native frog M ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.2399 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.410, 184.410, 184.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.730 - 1.160 |
| R-factor | 0.14726 |
| Rwork | 0.147 |
| R-free | 0.16023 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ka3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.994 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 21.730 | 21.730 | 1.220 |
| High resolution limit [Å] | 1.160 | 3.660 | 1.160 |
| Rmerge | 0.059 | 0.035 | 0.243 |
| Number of reflections | 86036 | ||
| <I/σ(I)> | 12.7 | 28.6 | 1.8 |
| Completeness [%] | 92.8 | 99.2 | 52.6 |
| Redundancy | 6 | 6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 1.6-2M magnesium chloride, 0.1M bicine , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
