4LQH
High resolution structure of native frog M ferritin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-18 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.2399 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 184.410, 184.410, 184.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.730 - 1.160 |
R-factor | 0.14726 |
Rwork | 0.147 |
R-free | 0.16023 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ka3 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.994 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 21.730 | 21.730 | 1.220 |
High resolution limit [Å] | 1.160 | 3.660 | 1.160 |
Rmerge | 0.059 | 0.035 | 0.243 |
Number of reflections | 86036 | ||
<I/σ(I)> | 12.7 | 28.6 | 1.8 |
Completeness [%] | 92.8 | 99.2 | 52.6 |
Redundancy | 6 | 6 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 1.6-2M magnesium chloride, 0.1M bicine , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |