4LQ4
crystal structure of mutant ribosomal protein L1 from Methanococcus jannaschii with deletion of 8 residues from C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-04-27 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54189 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.868, 105.255, 38.526 |
| Unit cell angles | 90.00, 104.01, 90.00 |
Refinement procedure
| Resolution | 32.860 - 1.750 |
| R-factor | 0.1673 |
| Rwork | 0.164 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i2a |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.051 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1389)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 26074 | |
| Completeness [%] | 98.8 | 93.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% PEG4000, 10% isopropanol, 0.1 M Hepes-NaOH, 0.5 mkl 0.8 KNa-tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
