4LQ1
Crystal Structure of E.Coli Branching Enzyme in complex with maltohexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-13 |
| Detector | MAR CCD |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.694, 104.113, 186.712 |
| Unit cell angles | 90.00, 91.85, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.550 |
| R-factor | 0.1826 |
| Rwork | 0.178 |
| R-free | 0.22380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 5.490 | 2.550 |
| Rmerge | 0.080 | 0.051 | 0.439 |
| Number of reflections | 111798 | ||
| <I/σ(I)> | 15.3 | ||
| Completeness [%] | 96.3 | 97.9 | 97.1 |
| Redundancy | 3.7 | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 0.1M HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
