4LQ1
Crystal Structure of E.Coli Branching Enzyme in complex with maltohexaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-13 |
Detector | MAR CCD |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.694, 104.113, 186.712 |
Unit cell angles | 90.00, 91.85, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.550 |
R-factor | 0.1826 |
Rwork | 0.178 |
R-free | 0.22380 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.550 | 5.490 | 2.550 |
Rmerge | 0.080 | 0.051 | 0.439 |
Number of reflections | 111798 | ||
<I/σ(I)> | 15.3 | ||
Completeness [%] | 96.3 | 97.9 | 97.1 |
Redundancy | 3.7 | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 0.1M HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |