4LQ0

Crystal structure of the I-LtrWI LAGLIDADG homing endonuclease bound to target DNA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	40.553, 71.959, 168.648
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.340 - 2.680
R-factor	0.20037
Rwork	0.197
R-free	0.27148
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qqy
RMSD bond length	0.011
RMSD bond angle	1.646
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (MR)
Refinement software	REFMAC (5.7.0025)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.500	2.910	2.800
High resolution limit [Å]	2.680	2.800	2.680
Number of reflections	13839
<I/σ(I)>	20	3.4	4.2
Completeness [%]	95.3	95	95
Redundancy	11.3	11.3	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	30% PEG MME 550 0.1 M Bis-Tris 50 mM Calcium Chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K